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KMID : 0352519800170030561
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Korea Univercity Medical Journal
1980 Volume.17 No. 3 p.561 ~ p.576
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The Study on Quality Control in Clinical Bacteriology
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郭賢淑/Kwak, Hyun-Sook
黃善哲/金仁仙/白承龍/Whang, Sun-Chul/Kim, In-Sun/Paik, Seung-Yong
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Abstract
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The clinical bacteriology concerns with the rapid and exact identification of pathogenic bacteria from various clinical specimens. To obtain the correct results, quality assurance program, such as continuing education of the laboratory worker, documentation of laboratory procedures, control of media, susceptibility test, equipment and collections of .stock organisms, and proficiency testing by government or any external agencies should be planned.
Author tried both internal and external quality control program in clinical bacteriology of Korea University Hospital and 10 general hospitals, using 10 kinds of standard ATCC registered strains and already identified clinical isolates. The organisms were introduced in the condition of imitate blind specimens of single ATCC strain, mixed ATCC strain, or -related species mixture.
In addition, the antimicrobial susceptibility test was performed on S. aureus and E. .coli ATCC strains.
The results were as follows:
1. The correct identification of single ATCC strain was initially 52% in Korea University Hospital and 4416 in 10 participating hospitals but the results improved to 82% and 70% in the fourth trials respectively.
2. The proficiencies of two strain mixture identification were less satisfactory than single strain identification and especially the identification of both organisms from two related species mixture was very poor.
3. The results of antimicrobial susceptibility test were relatively acceptable in S. aureus ATCC strain, but E. coli ATCC strain showed the results out of acceptable limits in Erythromycin, Chloramphenicol, Amphicillin and Cephalothin.
4. The proficiency according to the individual strain was about 60 61, but those of K. ozaenae, E. cloacae, and A. fecalis were less than 40%.
5. The proficiency of ATCC strains was high in E. coli, K. pneumoniae, and S. aureus, whereas the results of E. cloacae and S. marcescens were low.
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